ELISA kit for the quantitative determination of total aflatoxins in food samples.

Overall process times were geared to requirements as defined by the USDA GIPSA test performance specifications.

Method: Competitive enzyme immunoassay

Detection limit: 0.015 ppb
Wheat (0.9 ppb) - Barley (0.7 ppb) - Oats (0.8 ppb) - Rice (0.9 ppb) - Corn (1.1 ppb) - Beer (0.4 ppb) - Hazelnut ( 1.4 ppb) - Almond (1.2 ppb) - Chile (3 ppb) - Oil (0.4 ppb)

Quantification limit: 0,7 - 4,4 ppb

Assay time: 45 min

Detection: 450 nm

Recovery rate:
Wheat (99%) - Rye (92%) - Barley (92%) - Oats (85%) - Sorghum (101%) - Rice (102%) - Corn (92%) - Beer (101%) - Hazelnut ( 87%) - Peanut (101%) - Almond (93%) - Sultane (dry) (103%) - Fig (dry) (114%) - Chile (92%) - Oil (121%)

Standards: 0; 0,05; 0,1; 0,25; 0,5; 1,5 [ng/mL]
(1ng/mL = 1 000 ppt)

Ref. Range: 0 – 1,5 ppb

Cross reaction:
- Aflatoxin B1 100%
- Aflatoxin B2 73%
- Aflatoxin G1 74%
- Aflatoxin G2 49%
- Aflatoxin M1 60%


Storage temperature: 2 - 8ºC

Format: Microplate - 96 wells

- Quantitative results based on superior 4PL curve fitting for calibration
- Microsoft Excel-based calculation & evaluation tool available
- Reagents (excl. wash buffer) ready-to-use
- 96 well format with breakable microwell strips
- Simple extraction procedures
- Incubation at room temperature
- High accuracy and reproducibility
- High stability; minimum shelf life 12 months

Food/Feed samples